PUBLICATIONS
Below please find a list of relevant publications about the ORFV technology:
Efficient and scalable clarification of Orf virus from HEK suspension for vaccine development
F. Pagallies, J.J. Labisch, M. Wronska, K. Pflanz, R. Amann, Vaccine: X, Volume 18, 100474, June 2024.
An investigation of excipients for a stable Orf viral vector formulation
F. Eilts, Y.M.J. Harsy, K. Lothert, F. Pagallies, R. Amann, M.W. Wolff, Virus Research, Volume 336, 199213, 15 October 2023.
Stability studies for the identification of critical process parameters for a pharmaceutical production of the Orf virus
F. Eilts, J.J. Labisch, S. Orbay, Y.M.J. Harsy, M. Steger, F. Pagallies, R. Amann, K. Pflanz, M.W. Wolff, Vaccine, Volume 41, Issue 32, 19 July 2023, Pages 4731-4742.
A Summary of Practical Considerations for the Application of the Steric Exclusion Chromatography for the Purification of the Orf Viral Vector
F. Eilts, K. Lothert, S. Orbay, F. Pagallies, R. Amann, M.W. Wolff, Membranes (Basel),2022 Oct 29;12(11):1070.
Comparison of sample preparation techniques for the physicochemical characterization of Orf virus particles
F. Eilts, M. Steger, F. Pagallies, H.-J. Rziha, M. Hardt, R. Amann, M.W. Wolff, J Virol Methods, 2022 Dec;310:114614.
Orf Virus-Based Vectors Preferentially Target Professional Antigen-Presenting Cells, Activate the STING Pathway and Induce Strong Antigen-Specific T Cell Responses
M. Müller, A. Reguzova, M.W. Löffler, R. Amann, Frontiers in Immunology, 2022. 13.
A Novel Orf Virus D1701-VrV-Based Dengue Virus (DENV) Vaccine Candidate Expressing HLA-Specific T Cell Epitopes: A Proof-of-Concept Study
A. Reguzova, N. Fischer, M. Müller, F. Salomon, T. Jaenisch, R. Amann, Biomedicines, 9(12): 1862.
A scalable downstream process for the purification of the cell culture-derived Orf virus for human or veterinary applications.
K. Lothert, F. Pagallies, F. Eilts, R. Amann et al., Journal of Biotechnology, 323: 221-230.
Selection of chromatographic methods for the purification of cell culture-derived Orf virus for its application as a vaccine or viral vector.
K. Lothert, F. Pagallies, T. Feger, R. Amann, & M. W. Wolff, Journal of Biotechnology, 323: 62-72.
Orf Virus-Based Vaccine Vector D1701-V Induces Strong CD8+ T Cell Response against the Transgene but Not against ORFV-Derived Epitopes.
A. Reguzova, M. Ghosh, M. Müller, H.-J. Rziha & R. Amann, Vaccines, 8(2): 295.
An Orf Virus-based therapeutic vaccine for the treatment of papillomavirus-induced tumors.
M. Schneider, M. Müller, A. Yigitliler, R. Amann et al., Journal of Virology, 94(15): e00398-20.
Genomic Characterization of Orf Virus Strain D1701-V (Parapoxvirus) and Development of Novel Sites for Multiple Transgene Expression.
H.-J. Rziha, M. Büttner, M. Müller, F. Salomon, A. Reguzova, D. Laible & R. Amann, Viruses, 11(2): 127.
Generation and Selection of Orf Virus (ORFV) Recombinants Methods.
H.-J. Rziha, J. Rohde & R. Amann, Methods in Molecular Biology, 1349: 177-200.
Orf virus (ORFV) ANK-1 protein mitochondrial localization is mediated by ankyrin repeat motifs.
K. Lacek, B. Bauer, K. Bienkowska-Szewczyk & H.-J. Rziha, Virus Genes, 49 (1): 68-79.
New Orf Virus (Parapoxvirus) Recombinant Expressing H5 Hemagglutinin Protects Mice against H5N1 and H1N1 Influenza A Virus.
J. Rohde, R. Amann & H.-J. Rziha, PLoS One, 8(12):e83802.
A new rabies vaccine based on a recombinant ORF virus (parapoxvirus) expressing the rabies virus glycoprotein.
R. Amann, J. Rohde, U. Wulle , D. Conlee, R. Raue, O. Martinon & H.-J. Rziha, Journal of Virology, 87(3): 1618-30.
A new recombinant Orf virus (ORFV, Parapoxvirus) protects rabbits against lethal infection with rabbit hemorrhagic disease virus (RHDV).
J. Rohde, H. Schirrmeier, H. Granzow & H.-J. Rziha, Vaccine, 29(49): 9256-9264.
Comparison of different prime-boost regimes with DNA and recombinant Orf virus based vaccines expressing glycoprotein D of pseudorabies virus in pigs.
E.M.A. van Rooij, F.A.M. Rijsewijk, H.W. Moonen-Leusen, A.T.J. Bianchi & H.-J. Rziha, Vaccine, 28(7): 1808-1813.
New baculovirus recombinants expressing Pseudorabies virus (PRV) glycoproteins protect mice against lethal challenge infection.
A.K. Grabowska, A.D. Lipinska, J. Rohde, B. Szewczyk, K. Bienkowska-Szewczyk & H.-J. Rziha, Vaccine, 27(27): 3584-3591.
Prime-boost immunization using DNA vaccine and recombinant Orf virus protects pigs against Pseudorabies virus (Herpes suid 1).
D. Dory, V. T. Fischer, V. Beven, R. Cariolet, H.-J. Rziha & A. Jestin, Vaccine, 24(37-39): 6256-6263.
CD8 T cells require gamma interferon to clear Borna disease virus from the brain and prevent immune system-mediated neuronal damage.
J. Hausmann, A. Pagenstecher, K. Baur, H.-J. Rziha & P. Staeheli, Journal of Virology, 79(21): 13509-13518.
Prevention of virus persistence and protection against immunopathology after Borna disease virus infection of the brain by a novel Orf virus recombinant.
M. Henkel, O. Planz, T. Fischer, L. Stitz & H.-J. Rziha, Journal of Virology, 79(1): 314-325.
Appearance of the Bona Fide Spiral Tubule of Orf Virus Is Dependent on an Intact 10-Kilodalton Viral Protein.
D. Spehner, S. De Carlo, R. Drillien, F. Weiland, K. Mildner, D. Hanau & H.-J. Rziha, Journal of Virology, 78(15), 8085-8093.
Novel recombinant Parapoxvirus vectors induce protective humoral and cellular immunity against lethal herpesvirus challenge infection in mice.
T. Fischer, O. Planz, L. Stitz & H.-J. Rziha, Journal of Virology, 77(17): 9312-9323.
Relatedness and heterogeneity at the near-terminal end of the genome of a parapoxvirus bovis 1 strain (B177) compared with parapoxvirus ovis (Orf virus).
H.-J. Rziha, B. Bauer, K.-H. Adam, M. Röttgen, R. Cottone, M. Henkel, C. Dehio & M. Büttner, Journal of General Virology, 84(5): 1111-1116.
Parapoxviruses: From the Lesion to the Viral Genome.
M. Büttner & H.-J. Rziha, Journal of Veterinary Medicine Series B 49(1): 7-16.
Orf virus encodes a functional dUTPase gene.
R. Cottone, M. Büttner, C.J. McInnes, A.R. Wood & H.-J. Rziha, Journal of General Virology, 83(5): 1043-1048.
Generation of recombinant parapoxviruses: Non-essential genes suitable for insertion and expression of foreign genes.
H.-J. Rziha, M. Henkel, R. Cottone, B. Bauer, U. Auge, et al., Journal of Biotechnology, 83(1-2): 137-145.
A novel vascular endothelial growth factor encoded by Orf virus, VEGF-E, mediates angiogenesis via signalling by VEGFR-2 (KDR) but not VEGFR-1 (Flt-1) receptor tyrosine kinase.
M. Meyer, C. Lanz, M. Clauss, A. Lepple-Wienhaus, H.-J. Rziha et al., The EMBO Journal, 18(2): 363-374.
Parapoxviruses: potential alternative vectors for directing the immune response in permissive and non-permissive hosts.
H.-J. Rziha, M. Henkel, R. Cottone, M. Meyer, C. Dhio, C. & M. Büttner, Journal of Biotechnology, 73(2-3): 235-242.
Analysis of genomic rearrangement and subsequent gene deletion of the attenuated Orf virus strain D1701.
R. Cottone, M. Büttner, B. Bauer, M. Henkel, E. Hettich & H.-J. Rziha, Journal of Virology, Virus Research, 56(1): 53-67.
